1. Tripathi, A. K.; Ahamad, N.; Tyagi, A.; Quainoo, B.; Smith, A. W.; Singh, P.; Sabnis, N.; Vishwanatha, J. K. An MIEN1-Based Hexamer Peptide (LA3IK) Inhibits EGF-Driven Oncogenic Signaling in Prostate Cancer by Disrupting EGFR–ERBB2 Heterodimerization. Scientific Reports2026. https://doi.org/10.1038/s41598-026-41933-1.

  2. Smith, A. W.; Barrera, F. N. Regulation of Receptor Tyrosine Kinase Hetero-Interactions. Current Opinion in Structural Biology 2025, 95, 103187. https://doi.org/10.1016/j.sbi.2025.103187.

  3. Burns, E. A.; Matyas, B.; Lidke, D. S.; Smith, A. W. Building a Dynamic Model for EGFR-Family Dimerization Using Quantitative, Live-Cell Fluorescence Methods. Biophysical Journal 2025. https://doi.org/10.1016/j.bpj.2025.09.018.

  4. Singh, P. K.; Rybak, J. A.; Schuck, R. J.; Sahoo, A. R.; Buck, M.; Barrera, F. N.; Smith, A. W. Phosphatidylinositol 4,5-Bisphosphate Drives the Formation of EGFR and EphA2 Complexes. Science Advances 2024, 10 (49), eadl0649–eadl0649. https://doi.org/10.1126/sciadv.adl0649.

  5. Tydings, C. W.; Singh, B.; Smith, A. W.; Ledwitch, K. V.; Brown, B. P.; Lovly, C. M.; Walker, A. S.; Meiler, J. Analysis of EGFR Binding Hotspots for Design of New EGFR Inhibitory Biologics. Protein Science 2024, 33 (10). https://doi.org/10.1002/pro.5141.

  6. Singh, P. K.; Kim, S.; Smith, A. W. HER4 Is a High‐Affinity Dimerization Partner for All EGFR/HER/ErbB Family Proteins. Protein Science 2024, 33 (10). https://doi.org/10.1002/pro.5171.

  7. Smith, A. W. Recent Applications of Fluorescence Correlation Spectroscopy in Live Cells. Current Opinion in Chemical Biology 2024, 81, 102480–102480. https://doi.org/10.1016/j.cbpa.2024.102480.

  8. ‌‌Liang, J.; Smith, A. W. The Oligomeric State of Vasorin in the Plasma Membrane Measured Non-Invasively by Quantitative Fluorescence Fluctuation Spectroscopy. International Journal of Molecular Sciences 2024, 25 (7), 4115–4115. https://doi.org/10.3390/ijms25074115.

  9. Liang, J.; Seghiri, M.; Singh, P. K.; Seo, H. G.; Lee, J. Y.; Jo, Y.; Song, Y. B.; Park, C.; Zalicki, P.; Jeong, J.-Y.; Huh, W.-K.; Caculitan, N. G.; Smith, A. W. The β2-Adrenergic Receptor Associates with CXCR4 Multimers in Human Cancer Cells. Proceedings of the National Academy of Sciences 2024, 121 (14). https://doi.org/10.1073/pnas.2304897121.

  10. Villaber, R. A.; Singh, P. K.; Smith, A. Abstract 1903 Epidermal Growth Factor Receptor Interactions with Phosphatidylinositol 4,5-Bisphosphate. Journal of Biological Chemistry 2024, 300 (3), 106380. https://doi.org/10.1016/j.jbc.2024.106380.

  11. Smith, A.; Singh, P. Abstract 1922 Probing the Degree of Interaction between HER4 and Other HER/ErbB-Family Proteins in Live Cells. Journal of Biological Chemistry 2024, 300 (3), 107005. https://doi.org/10.1016/j.jbc.2024.107005.

  12. Singh, P. K.; Smith, A. W. HER4 and Its Interaction with Her Family Members: PIE-FCCS Study. Biophysical Journal 2024, 123 (3), 481a. https://doi.org/10.1016/j.bpj.2023.11.2919.

  13. Rybak, J. A.; Singh, P. K.; Schuck, R. J.; Barrera, F. N.; Smith, A. W. Investigating the Effect of PIP2 on the Homo- and Hetero-Oligomerization of EGFR and EphA2. Biophysical Journal 2024, 123 (3), 451a. https://doi.org/10.1016/j.bpj.2023.11.2749.

  14. Shi, X.; Lingerak, R.; Herting, C. J.; Ge, Y.; Kim, S.; Toth, P.; Wang, W.; Brown, B. P.; Meiler, J.; Sossey-Alaoui, K.; et al. Time-resolved live-cell spectroscopy reveals EphA2 multimeric assembly. Science 2023, 382 (6674), 1042-1050. DOI: https://doi.org/10.1126/science.adg5314.

  15. Shi, X.; Lingerak, R.; Shrestha, P.; Buck, M.; Wang, B.-C.; Smith, A. W. The SAM Domain of EphA2 Inhibits Ligand-Independent Clustering and Activation. bioRxiv 2023, 2023.2010.2005.561050. DOI: https://doi.org/10.1101/2023.10.05.561050.

  16. Rybak, J. A.; Sahoo, A. R.; Kim, S.; Pyron, R. J.; Pitts, S. B.; Guleryuz, S.; Smith, A. W.; Buck, M.; Barrera, F. N. Allosteric inhibition of the epidermal growth factor receptor through disruption of transmembrane interactions. J Biol Chem 2023, 104914. DOI: https://doi.org/10.1016/j.jbc.2023.104914.

  17. Mallory, D. P.; Freedman, A.; Kaliszewski, M. J.; Montenegro-Galindo, G. R.; Pugh, C.; Smith, A. W. Direct Quantification of Serum Protein Interactions with PEGylated Micelle Nanocarriers. Biomacromolecules 2023, 24 (6), 2479-2488. DOI: https://doi.org/10.1021/acs.biomac.2c01538.

  18. Brown, B. P.; Zhang, Y. K.; Kim, S.; Finneran, P.; Yan, Y.; Du, Z.; Kim, J.; Hartzler, A. L.; LeNoue-Newton, M. L.; Smith, A. W.; et al. Allele-specific activation, enzyme kinetics, and inhibitor sensitivities of EGFR exon 19 deletion mutations in lung cancer. Proc Natl Acad Sci USA 2022, 119 (30), e2206588119. DOI: https://doi.org/10.1073/pnas.2206588119.

  19. Feng, J.; Khakipoor, B.; May, J.; Mulford, M.; Davis, J.; Siman, K.; Russell, G.; Smith, A. W.; King, H. An open-source dual-beam spectrophotometer for citizen-science-based water quality monitoring. HardwareX 2021, 10, e00241. DOI: https://doi.org/10.1016/j.ohx.2021.e00241.

  20. Du, Z.; Brown, B. P.; Kim, S.; Ferguson, D.; Pavlick, D. C.; Jayakumaran, G.; Benayed, R.; Gallant, J. N.; Zhang, Y. K.; Yan, Y.; et al. Structure-function analysis of oncogenic EGFR Kinase Domain Duplication reveals insights into activation and a potential approach for therapeutic targeting. Nat Commun 2021, 12 (1), 1382. DOI: https://doi.org/10.1038/s41467-021-21613-6.

  21. Christie, S. M.; Hao, J.; Tracy, E.; Buck, M.; Yu, J. S.; Smith, A. W. Interactions between semaphorins and plexin-neuropilin receptor complexes in the membranes of live cells. J Biol Chem 2021, 297 (2), 100965. DOI: https://doi.org/10.1016/j.jbc.2021.100965.

  22. Asher, W. B.; Geggier, P.; Holsey, M. D.; Gilmore, G. T.; Pati, A. K.; Meszaros, J.; Terry, D. S.; Mathiasen, S.; Kaliszewski, M. J.; McCauley, M. D.; et al. Single-molecule FRET imaging of GPCR dimers in living cells. Nat Methods 2021, 18 (4), 397-405. DOI: https://doi.org/10.1038/s41592-021-01081-y.

  23. Ge, Y.; Shi, X.; Boopathy, S.; McDonald, J.; Smith, A. W.; Chao, L. H. Two forms of Opa1 cooperate to complete fusion of the mitochondrial inner-membrane. eLife 2020, 9. DOI: https://doi.org/10.7554/eLife.50973.

  24. Ge, Y.; Boopathy, S.; Smith, A.; Chao, L. H. A model membrane platform for reconstituting mitochondrial membrane dynamics. J Vis Exp 2020,  (163). DOI: https://doi.org/10.3791/61620.

  25. Christie, S. M.; Ham, T. R.; Gilmore, G. T.; Toth, P. D.; Leipzig, N. D.; Smith, A. W. Covalently immobilizing interferon-γ drives filopodia Production through Specific Receptor-Ligand Interactions Independently of Canonical Downstream Signaling. Bioconjug Chem 2020, 31 (5), 1362-1369. DOI: https://doi.org/10.1021/acs.bioconjchem.0c00105.

  26. Christie, S.; Shi, X.; Smith, A. W. Resolving membrane protein-protein interactions in live cells with pulsed interleaved excitation fluorescence cross-correlation spectroscopy. Acc Chem Res 2020, 53 (4), 792-799. DOI: https://doi.org/10.1021/acs.accounts.9b00625.

  27. Li, X.; Smith, A. W. Quantifying Lipid Mobility and Peptide Binding for Gram-Negative and Gram-Positive Model Supported Lipid Bilayers. J Phys Chem B 2019, 123 (49), 10433-10440. DOI: https://doi.org/10.1021/acs.jpcb.9b09709.

  28. Li, X.; Shi, X.; Kaliszewski, M. J.; Smith, A. W. Chapter Three - Fluorescence cross-correlation spectroscopy of lipid-peptide interactions on supported lipid bilayers. Adv Biomem and Lip Self-Assem 2019, 29, 49-68. DOI: https://doi.org/10.1016/bs.abl.2019.01.005.

  29. Bogucki, R.; Greggila, M.; Mallory, P.; Feng, J.; Siman, K.; Khakipoor, B.; King, H.; Smith, A. W. A 3D-Printable Dual Beam Spectrophotometer with Multiplatform Smartphone Adaptor. J Chem Edu 2019, 96 (7), 1527-1531. DOI: https://doi.org/10.1021/acs.jchemed.8b00870.

  30. Agarwal, G.; Smith, A. W.; Jones, B. Discoidin domain receptors: Micro insights into macro assemblies. Biochim Biophys Acta Mol Cell Res 2019, 1866 (11), 118496. DOI: https://doi.org/10.1016/j.bbamcr.2019.06.010.

  31. Mallory, D. P.; Gutierrez, E.; Pinkevitch, M.; Klinginsmith, C.; Comar, W. D.; Roushar, F. J.; Schlebach, J. P.; Smith, A. W.; Jastrzebska, B. The Retinitis Pigmentosa-Linked Mutations in Transmembrane Helix 5 of Rhodopsin Disrupt Cellular Trafficking Regardless of Oligomerization State. Biochemistry 2018, 57 (35), 5188-5201. DOI: https://doi.org/10.1021/acs.biochem.8b00403.

  32. Kaliszewski, M. J.; Shi, X.; Hou, Y.; Lingerak, R.; Kim, S.; Mallory, P.; Smith, A. W. Quantifying membrane protein oligomerization with fluorescence cross- correlation spectroscopy. Methods 2018, 140-141, 40-51. DOI: https://doi.org/10.1016/j.ymeth.2018.02.002.

  33. Alves, D. S.; Westerfield, J. M.; Shi, X.; Nguyen, V. P.; Stefanski, K. M.; Booth, K. R.; Kim, S.; Morrell-Falvey, J.; Wang, B.-C.; Abel, S. M.; et al. A novel pH-dependent membrane peptide that binds to EphA2 and inhibits cell migration. eLife 2018, 7, e36645. DOI: https://doi.org/10.7554/eLife.36645.

  34. Zeng, Z.; Shi, X.; Mabe, T.; Christie, S.; Gilmore, G.; Smith, A. W.; Wei, J. Protein Trapping in Plasmonic Nanoslit and Nanoledge Cavities: The Behavior and Sensing. Anal Chem 2017, 89 (10), 5221-5229. DOI: https://doi.org/10.1021/acs.analchem.6b04493.

  35. Shi, X.; Hapiak, V.; Zheng, J.; Muller-Greven, J.; Bowman, D.; Lingerak, R.; Buck, M.; Wang, B. C.; Smith, A. W. A role of the SAM domain in EphA2 receptor activation. Sci Rep 2017, 7, 45084. DOI: https://doi.org/10.1038/srep45084.

  36. Jastrzebska, B.; Comar, W. D.; Kaliszewski, M. J.; Skinner, K. C.; Torcasio, M. H.; Esway, A. S.; Jin, H.; Palczewski, K.; Smith, A. W. A G Protein-Coupled Receptor Dimerization Interface in Human Cone Opsins. Biochemistry 2017, 56 (1), 61-72. DOI: https://doi.org/10.1021/acs.biochem.6b00877.

  37. Smith, A. W.; Huang, H. H.; Endres, N. F.; Rhodes, C.; Groves, J. T. Dynamic Organization of Myristoylated Src in the Live Cell Plasma Membrane. J Phys Chem B 2016, 120 (5), 867-876. DOI: https://doi.org/10.1021/acs.jpcb.5b08887.

  38. Shi, X.; Kohram, M.; Zhuang, X.; Smith, A. W. Interactions and Translational Dynamics of Phosphatidylinositol Bisphosphate (PIP2) Lipids in Asymmetric Lipid Bilayers. Langmuir 2016, 32 (7), 1732-1741. DOI: https://doi.org/10.1021/acs.langmuir.5b02814.

  39. Liu, C.; Wang, K.; Yi, C.; Shi, X.; Smith, A. W.; Gong, X.; Heeger, A. J. Efficient Perovskite Hybrid Photovoltaics via Alcohol-Vapor Annealing Treatment. Adv Func Mater 2016, 26 (1), 101-110. DOI: https://doi.org/10.1002/adfm.201504041.

  40. Huang, Y.; Bharill, S.; Karandur, D.; Peterson, S. M.; Marita, M.; Shi, X.; Kaliszewski, M. J.; Smith, A. W.; Isacoff, E. Y.; Kuriyan, J. Molecular basis for multimerization in the activation of the epidermal growth factor receptor. eLife 2016, 5, e14107. DOI: https://doi.org/10.7554/eLife.14107.

  41. Grasse, E. K.; Torcasio, M. H.; Smith, A. W. Teaching UV–Vis Spectroscopy with a 3D-Printable Smartphone Spectrophotometer. J Chem Edu 2016, 93 (1), 146-151. DOI: https://doi.org/10.1021/acs.jchemed.5b00654.

  42. Smith, A. W. Detection of rhodopsin dimerization in situ by PIE-FCCS, a time-resolved fluorescence spectroscopy. Methods Mol Biol 2015, 1271, 205-219. DOI: https://doi.org/10.1007/978-1-4939-2330-4_14.

  43. Shi, X.; Li, X.; Kaliszewski, M. J.; Zhuang, X.; Smith, A. W. Tuning the Mobility Coupling of Quaternized Polyvinylpyridine and Anionic Phospholipids in Supported Lipid Bilayers. Langmuir 2015, 31 (5), 1784-1791. DOI: https://doi.org/10.1021/la504241w.

  44. Marita, M.; Wang, Y.; Kaliszewski, Megan J.; Skinner, Kevin C.; Comar, William D.; Shi, X.; Dasari, P.; Zhang, X.; Smith, Adam W. Class A Plexins Are Organized as Preformed Inactive Dimers on the Cell Surface. Biophys J 2015, 109 (9), 1937-1945. DOI: https://doi.org/10.1016/j.bpj.2015.04.043.

  45. Liu, C.; Wang, K.; Yi, C.; Shi, X.; Du, P.; Smith, A. W.; Karim, A.; Gong, X. Ultrasensitive solution-processed perovskite hybrid photodetectors. Journal of Materials Chemistry C 2015, 3 (26), 6600-6606. DOI: https://doi.org/10.1039/C5TC00673B.

  46. Biswas, K. H.; Hartman, K. L.; Yu, C.-h.; Harrison, O. J.; Song, H.; Smith, A. W.; Huang, W. Y. C.; Lin, W.-C.; Guo, Z.; Padmanabhan, A.; et al. E-cadherin junction formation involves an active kinetic nucleation process. Proc Natl Acad Sci USA 2015, 112 (35), 10932-10937. DOI: https://doi.org/10.1073/pnas.1513775112.

  47. Comar, W. D.; Schubert, S. M.; Jastrzebska, B.; Palczewski, K.; Smith, A. W. Time-Resolved Fluorescence Spectroscopy Measures Clustering and Mobility of a G Protein-Coupled Receptor Opsin in Live Cell Membranes. J Am Chem Soc 2014, 136 (23), 8342-8349. DOI: https://doi.org/10.1021/ja501948w.

  48. Smoligovets, Alexander A.; Smith, Adam W.; Groves, Jay T. Ratiometric Imaging of the T-Cell Actin Cytoskeleton Reveals the Nature of Receptor-Induced Cytoskeletal Enrichment. Biophys J 2013, 105 (3), L11-L13. DOI: https://doi.org/10.1016/j.bpj.2013.06.031.

  49. Endres, N. F.; Das, R.; Smith, A. W.; Arkhipov, A.; Kovacs, E.; Huang, Y.; Pelton, J. G.; Shan, Y.; Shaw, D. E.; Wemmer, D. E.; et al. Conformational coupling across the plasma membrane in activation of the EGF receptor. Cell 2013, 152 (3), 543-556. DOI: https://doi.org/10.1016/j.cell.2012.12.032.

  50. Triffo, S. B.; Huang, H. H.; Smith, A. W.; Chou, E. T.; Groves, J. T. Monitoring lipid anchor organization in cell membranes by PIE-FCCS. J Am Chem Soc 2012, 134 (26), 10833-10842. DOI: https://doi.org/10.1021/ja300374c.

  51. Smoligovets, A. A.; Smith, A. W.; Wu, H. J.; Petit, R. S.; Groves, J. T. Characterization of dynamic actin associations with T-cell receptor microclusters in primary T cells. J Cell Sci 2012, 125 (Pt 3), 735-742. DOI: https://doi.org/10.1242/jcs.092825.

  52. Smith, A. W. Lipid-protein interactions in biological membranes: a dynamic perspective. Biochim Biophys Acta 2012, 1818 (2), 172-177. DOI: https://doi.org/10.1016/j.bbamem.2011.06.015.

  53. Belardi, B.; O’Donoghue, G. P.; Smith, A. W.; Groves, J. T.; Bertozzi, C. R. Investigating Cell Surface Galectin-Mediated Cross-Linking on Glycoengineered Cells. J Am Chem Soc 2012, 134 (23), 9549-9552. DOI: https://doi.org/10.1021/ja301694s.

  54. Smith, A. W.; Smoligovets, A. A.; Groves, J. T. Patterned Two-Photon Photoactivation Illuminates Spatial Reorganization in Live Cells. J Phys Chem A 2011, 115 (16), 3867-3875. DOI: https://doi.org/10.1021/jp108295s.

  55. Smith, A. W.; Lessing, J.; Ganim, Z.; Peng, C. S.; Tokmakoff, A.; Roy, S.; Jansen, T. L. C.; Knoester, J. Melting of a β-Hairpin Peptide Using Isotope-Edited 2D IR Spectroscopy and Simulations. J Phys Chem B 2010, 114 (34), 10913-10924. DOI: https://doi.org/10.1021/jp104017h.

  56. Ganim, Z.; Chung, H. S.; Smith, A. W.; DeFlores, L. P.; Jones, K. C.; Tokmakoff, A. Amide I Two-Dimensional Infrared Spectroscopy of Proteins. Acc Chem Res 2008, 41 (3), 432-441. DOI: https://doi.org/10.1021/ar700188n.

  57. Smith, A. W.; Tokmakoff, A. Probing local structural events in β-hairpin unfolding with transient nonlinear infrared spectroscopy. Angew Chem Int Ed Engl 2007, 46 (42), 7984-7987. DOI: https://doi.org/10.1002/anie.200701172.

  58. Smith, A. W.; Tokmakoff, A. Amide I two-dimensional infrared spectroscopy of β-hairpin peptides. J Chem Phys 2007, 126 (4), 045109. DOI: https://doi.org/10.1063/1.2428300.

  59. Smith, A. W.; Chung, H. S.; Ganim, Z.; Tokmakoff, A. Multidimensional IR Spectroscopy of Site-Specific Hairpin Folding. In Ultrafast Phenom XV, Berlin, Heidelberg, 2007; Corkum, P., Jonas, D. M., Miller, R. J. D., Weiner, A. M., Eds.; Springer Berlin Heidelberg: pp 350-352. DOI: https://doi.org/10.1007/978-3-540-68781-8_113.

  60. Chung, H. S.; Khalil, M.; Smith, A. W.; Tokmakoff, A. Transient two-dimensional IR spectrometer for probing nanosecond temperature-jump kinetics. Rev Sci Instrum 2007, 78 (6), 063101. DOI: https://doi.org/10.1063/1.2743168.

  61. Smith, A. W.; Chung, H. S.; Ganim, Z.; Tokmakoff, A. Residual native structure in a thermally denatured β-hairpin. J Phys Chem B 2005, 109 (36), 17025-17027. DOI: https://doi.org/10.1021/jp053949m.

  62. Chung, H. S.; Khalil, M.; Smith, A. W.; Ganim, Z.; Tokmakoff, A. Conformational changes during the nanosecond-to-millisecond unfolding of ubiquitin. Proc Natl Acad Sci USA 2005, 102 (3), 612-617. DOI: https://doi.org/10.1073/pnas.0408646102.

  63. Sickafoose, S. M.; Smith, A. W.; Morse, M. D. Optical spectroscopy of tungsten carbide (WC). The Journal of Chemical Physics 2002, 116 (3), 993-1002. DOI: https://doi.org/10.1063/1.1427068.